Resveratrol Stabilizes Cytosolic ß-Catenin Through The Activation of The Thermogenic Cascade

Purpose: Resveratrol is a polyphenolic compound that has been shown to stimulate fatty acid oxidation and increase the expression of genes associated with thermogenic processes. Therefore, it is important to investigate the effects of resveratrol beyond intracellular thermogenic pathways to determine side effects at the cellular level. The aim of this study was to evaluate the effect of resveratrol on intracellular ß-catenin, in addition to its thermogenic effect. Materials and methods: The effect of resveratrol on thermogenic genes such as SIRT1 gene, UCP1, PGC1α, PRDM16 and ß-catenin gene expression levels was analyzed by RT-PCR. The cytosolic ß-catenin level analyzed by western-blot. Results: The effect of resveratrol on thermogenic genes such as SIRT1 gene, UCP1, PGC1α, PRDM16 and ß-catenin gene expression levels was analyzed by RT-PCR. The cytosolic ß-catenin level analyzed by western-blot. We found that the expression levels of UCP1, PPARγ, PRDM16 were increased by SIRT1 protein activated by resveratrol, but it had no effect on ß-catenin mRNA expression. While ß-catenin did not change at the mRNA level, we found that ß-catenin increased at the protein level in the cell independently of the canonical Wnt pathway. Conclusion: Our results suggest that resveratrol, which is widely used as a supplement, may also act on different signaling pathways in the cell.