Figure S5 from Estrogen Receptor Blockade Potentiates Immunotherapy for Liver Metastases by Altering the Liver Immunosuppressive Microenvironment

Macrophages analyses. Shown in (a) are the M1:M2 macrophages ratio analysis results using additional CD38 marker (n=3-4) and (b) TNF-alpha for M1-like macrophages characterization, and the use of the additional OVX+E2 experimental group which shows a reverse trend upon estrogen supplementation compared to the OVX group (n=3-4). Shown in (c) are the macrophages flow cytometry analysis results in FC1199 pancreatic liver metastasis model at Day 12 post-tumor injection (n=4).