Figure 3 from Estrogen Receptor Blockade Potentiates Immunotherapy for Liver Metastases by Altering the Liver Immunosuppressive Microenvironment

Increased CXCR3 expression in hepatic macrophages associated with early stages of the metastatic process. FC was conducted on hepatic immune cells from mice injected with 5 × 10⁵ MC-38 cells 8 days earlier. Shown in A–D are FC results for the indicated cell populations (n = 3–4). Confocal images (E, F, H) were acquired for 10 μm liver cryostat sections from these mice that were immunostained with the indicated antibodies and counterstained with 4′,6-diamidino-2-phenylindole. Cell counts per field (means ± SD, n = 10) are shown on the right. Representative RNAscope images were obtained from cryostat sections probed for CXCL10 expression in NKT (G) or macrophages (I), as indicated. Image analysis (G, I, right) was based on 15 fields per group (n = 2–3). Yellow arrows denote NK1.1⁺ or CD68⁺ cells expressing CXCL10, and green arrows denote cells with high CXCL10 expression. *, P ≤ 0.05; **, P ≤ 0.01; ***, P ≤ 0.001; ****, P ≤ 0.0001, Student t test.