Figure 7 from Estrogen Receptor Blockade Potentiates Immunotherapy for Liver Metastases by Altering the Liver Immunosuppressive Microenvironment

The combinatorial Fulv and anti-PD-1 immunotherapy reprograms the IME of CRCLM. Hepatic immune cells were isolated following the treatment regimen depicted in Fig. 6A. Shown in A–J are results of FC performed on cells from the indicated treatment groups (n = 4). Shown in K and L are results of RT-qPCR performed on whole liver RNA (n = 3), normalized to GAPDH, and expressed as means (± SD) fold change relative to vehicle (assigned a value of 1). *, P ≤ 0.05; **, P ≤ 0.01; ***, P ≤ 0.001, Student t test.