Figure 3 from A Drug Discovery Pipeline for MAPK/ERK Pathway Inhibitors in Caenorhabditis elegans

Readouts for toxicity and MAPK/ERK pathway inhibition from fluorescence-based scoring. Heatmaps showing means from three independent experiments for each drug and concentration. A, Putative toxicity measured as percent larvae at time of analysis. B, MAPK/ERK pathway inhibition as mean number of vulvae per adult worm. C, Assay screening performance, estimated by Z-factors. *Dabrafenib and vemurafenib were assayed up to 20 μmol/L instead of 50 μmol/L, which was nonpermissive for larval growth.