Figure 4 from A Drug Discovery Pipeline for MAPK/ERK Pathway Inhibitors in Caenorhabditis elegans

Evaluation of pathway specificity. A, Differential interference contrast (DIC) micrographs showing overall body morphology (inlayed panels) and vulval phenotypes in untreated lin-15(n765) and lin-1(e1777) mutant adults. Arrows indicate functional vulvae, and arrowheads indicate pseudovulvae. Scale bars, 50 μm. B, Graphs indicating vulvae per adult scores (black squares) and Z-factors (red dots) after drug treatment in ST65, lin-15(n765), and lin-1(e1777) animals. Drug concentrations in μmol/L are indicated on the X-axis, the mean number of vulvae per adult on the left Y-axis, and Z-factors on the right Y-axis. Error bars show ± SDs from three independent experiments.