Figure 5 from A Drug Discovery Pipeline for MAPK/ERK Pathway Inhibitors in Caenorhabditis elegans

In silico scoring pipelines. A,In silico scoring of fluorescent foci using MetaXpress and CellProfiler software. From left, brightfield images, GFP images, and the concomitant detection mask that shows each detected feature in different colors (scale bars, 1 mm). Enlarged panels to the right show examples of what each program scores as adult pharynges, larval pharynges, and vulvae (scale bars, 100 μm). B–D, Boxplots showing scoring of the number of vulvae per adult worm after 10 μmol/L drug exposure. Boxes represent minimum to maximum distributions of three independent experiments with a line at the median from (B) manual scoring, (C) MetaXpress, and (D) CellProfiler pipelines. E, Violin plot comparing the number of reported vulvae per adult at 10 μmol/L drug exposure from MetaXpress, CellProfiler, and manual scoring protocols. F and G, Regression analysis of the number of reported vulvae per adult at 10 μmol/L drug exposure using (F) MetaXpress vs. manual scoring pipelines and (G) CellProfiler vs. manual scoring pipelines.