Supplementary Figure S10 from Single-cell RNA analysis reveals cell-intrinsic functions of CAR-T cells correlating with response in a phase II study of lymphoma patients

Supplementary Figure S10. A) Overview of the CAR-T production process. B) Expansion of the CAR-T product over time for responders (R; n=9) and non-responders (NR; n=15) during ex vivo CAR-T manufacturing. Values are shown as mean ± SEM. Two-way ANOVA with Sidak’s correction for multiple comparison was used to compare between groups. C) Days of ex vivo culture during CAR-T manufacturing from R (n=9) and NR (n=15). Each dot represent data from one patient and values are shown as mean ± SEM. Unpaired t-test was used to compare between groups. D) Correlation between the highest CAR copy number (peak CAR copies) detected in patient blood upon CAR-T infusion and days of ex-vivo CART expansion during manufacturing. Each dot represent data from one patient. E) Correlation between the highest CAR copy number (peak CAR copies) detected in patient blood upon CAR-T infusion and fold ex vivo expansion. F) Schematic illustration describing the analysis of how genes upregulated in non-responding patients (NR) change in expression (up- or down-regulated) with culture time. G) Genes with negative (downregulated) and positive (upregulated) correlation with culture time were compared with the genes specifically upregulated in CD8+ CAR-Ts from non-responding patients.