Figure 5 from Activation of Cell-Intrinsic Signaling in CAR-T Cells via a Chimeric IL7R Domain

Hinge modifications do not improve antigen specificity of CD123-targeted chimeric IL7Rα. A, Amino acid sequences of hinge regions used in functional studies. B, Transduction efficiency measured using his-tagged rhCD123 binding of engineered receptors and flow cytometry. n = 3 unique T-cell donors. C, Quantification of phosphorylated STAT5 percentage in CCR-T cells measured with and without activation on the immobilized rhCD123 target. n = 3 unique T-cell donors, comparison between pSTAT5% in cytokine-starved cells with or without rhCD123 activation; *, P D, Representative Western blot testing IgG4 vs. IH STAT5 activation with and without immobilized rhCD123. NT, nontransduced.