Figure 3 from ANGPTL4 Suppresses Clear Cell Renal Cell Carcinoma via Inhibition of Lysosomal Acid Lipase

nANGPTL4 suppresses ccRCC colony formation. A, MTS assay of the indicated CAKi-1 cells. Graph depicts the average absorbance ± SD. Two-way ANOVA was done to determine significance (n = 6 all groups). B, The indicated 786O cells were incubated with CSFE and analyzed by flow cytometry. The graph depicts the average % proliferation of the cells ± SD. Welch’s t test was done to determine significance. C, Soft agar colony assay. The indicated CAKi-1 cells were cultured on soft agar for 30 days and the number of colonies per well was counted. Left: representative images of colonies from the indicated cells (10× magnification). Right: graph depicts the average number of colonies ± SD. Welch’s t test was used to determine significance. D, The indicated RCC4 cell line was incubated in nonadherent conditions for 72 hours. The graph depicts the average number of colonies/well ± SD. Welch’s t test was done to determine significance. E, Graph depicts the average number of colonies/well of the indicated CAKi-1 cells treated as indicated with rat IgG or anti-cANGPTL4 ± SD. One-way ANOVA with Dunnett’s test to correct for multiple comparisons was done to determine significance. F, Graph depicts the average number of colonies per well ± SD. of CAKi-1 A4 KO cells transfected with full length (FL), N-terminus or mock transfected as indicated. One-way ANOVA with Dunnett’s test to correct for multiple comparisons was done to determine significance. G, Graph depicts the average number of colonies/well of the indicated 786O cells ± SD. Welch’s t test was done to determine significance. H, Graph depicts the average number of colonies/well ± SD of 786O A4 KO cells transfected with N-terminus or mock transfected as indicated. One-way ANOVA with Dunnett’s test to correct for multiple comparisons was done to determine significance.