Figure 1 from ANGPTL4 Suppresses Clear Cell Renal Cell Carcinoma via Inhibition of Lysosomal Acid Lipase

Subset of patients with ccRCC with low expression of ANGPTL4 is associated with shorter survival and lipid metabolism. A,ANGPTL4 expression in the indicated cancer types. Data generated from TCGA pan-cancer normalized RNAseq data generated using the Human Protein Atlas. B,ANGPTL4 mRNA expression in TCGA ccRCC samples compared to normal kidney tissue samples. C,ANGPTL4 expression in the indicated human ccRCC cell lines or immortalized human proximal tubule cells (HK-2) relative to GAPDH. D, Distribution of ANGPTL4 mRNA levels in TCGA ccRCC (KIRC) samples. Graph depicts the number of samples within the indicated robust Z-score range. A subset of patients with significantly lower ANGPTL4 expression (robust Z-score ≤ −3.5) is indicated as ANGPTL4-low distinguishable from the rest of the samples labeled as bulk samples. E, Kaplan–Meier curve depicting overall survival (OS) of ccRCC samples with low expression of ANGPTL4 compared to the rest of the sample. F, GSEA of TCGA samples comparing ANGPTL4-low (robust Z ≤ −3.5) and bulk samples (robust Z > −3.5). Graph depicts the nominal enrichment score (NES) of indicated gene sets enriched in the indicated group. The FDR is indicated. G and H, Enrichment plot for the indicated gene set from the GSEA in F.