Figure 2 from ANGPTL4 Suppresses Clear Cell Renal Cell Carcinoma via Inhibition of Lysosomal Acid Lipase

ANGPTL4 suppresses tumor growth of RCC cells with wild-type VHL. A, Graph depicts expression of ANGPTL4 in CAKi-1 WT or A4KO cells relative to PPIA as a fold change compared to WT. B, 2 × 10⁶ of the indicated CAKi-1 cells were implanted into the flank of NOD-SCID/IL2RG (NSG) mice. Graph depicts the average tumor volume ± SEM. Two-way ANOVA was done to determine significance (n = 8 for both groups). C, Renca mouse RCC cells were stably selected for expression of human ANGPTL4-V5. Immunoblot for V5 in the indicated Renca cells. D, Renca or Renca cells expressing human ANGPTL4-V5 (Renca-A4) from (C) were implanted into the mammary gland of Balb/C mice. Graph depicts the average tumor volume ± SEM. Two-way ANOVA was done to determine significance (n = 8 WT, n = 7 Renca-A4). E, 5 × 10⁶ of CAKi-1 cells were implanted into the flank of NSG mice. CAKi-1 tumor–bearing mice were treated with 200 μg anti-cANGPTL4 antibody or rat IgG isotype control twice weekly starting at day 14. Graph depicts the average tumor volume ± SEM. F, Graph depicts the number of CD31⁺/CD34⁺ endothelial cells ± SD as a % of single cells from the indicated tumor from (B). Welch’s t test was done to determine significance. G, Tumor sections from the indicated tumors in B, were stained for CD31. Graph depicts the average CD31⁺ staining as a % of total area ± SD. At least three images from three samples were used for each group. Welch’s t test was done to determine significance.