Figure 4 from ANGPTL4 Suppresses Clear Cell Renal Cell Carcinoma via Inhibition of Lysosomal Acid Lipase

Loss of ANGPTL4 is associated with enrichment for lipid metabolism genes. A, Volcano plot for CAKi1 A4KO versus WT cells. Genes with an absolute value log₂ fold change ≥1 and an adjusted P value of ≤0.05 are indicated. Genes upregulated in A4 KO cells are red and genes downregulated in A4KO cells are blue. B, GSEA was performed for CAKi-1 A4KO and WT cells. Graph depicts the nominal enrichment score (NES) of gene sets related to metabolism that were enriched—had a FDR <0.25—with A4KO cells. The nominal P value is indicated by the heat bar. Gray bars had a nominal P value that was lower than the threshold for the package used and thus had a value of 0. C, Enrichment plot for the indicated gene set from the GSEA analysis in B. The FDR and nominal P value are indicated. D, Heatmap of the genes in the gene set from C, for CAKi-1 A4KO and WT cells. E, GSEA analysis was performed for 786O A4KO and WT cells. Enrichment plot for the indicated gene set showing enrichment in A4KO cells. The FDR and nominal P value are indicated. F, Graphs depict the average mRNA expression relative to PPIA of the indicated genes in CAKi-1 WT and A4KO cells ± SD. Welch’s t test was done to determine significance.