Development of a MR-compatible bioreactor for 3D cell structure metabolic analysis in real-time for a 60 MHz benchtop NMR spectrometer.

This is the authors' abstract. We don't add key points for this paper.

Bioreactor platforms have been used in HP-NMR applications to analyze cellular metabolism in real-time, however they require complex set-ups and automated controlled systems, limiting their use. We present a system that overcomes these limitations consisting of a 3D printed platform, an NMR compatible 3D cell model and a recirculation circuit for single-shot and longitudinal hyperpolarization-enhanced NMR experiments. The platform presents no signal interference, a media system for biological support, and a straightforward and simplified manufacturing and assembly process. As a proof-of-concept, we present preliminary metabolic data of a 3D HeLa cell model analyzed with our bioreactor.