Supplementary Figure S16 from Overexpression of an Engineered SERPINB9 Enhances Allogeneic T-cell Persistence and Efficacy

Supplementary Figure S16. Effects of SB9 overexpression on the exhaustion status of CD30.CAR with 4-1BB spacer (CD30.bb) or CD19.CAR TCRαβKO ATCs following sequential encounters with tumor cells as described in Fig. 5A. Effector CAR T cells were co-cultured with HLADKO NALM6-CD30 tumor cells, at a fixed ratio of 1:1 or 1:5, when CD30.bb TCRKO ATCs (A) or CD19.CAR TCRKO ATCs (B) were studied. PD-1, TIM-3 and LAG-3 expression on effector cells before the start of co-culture (Day 0) and on the last day of co-culture.