Figure 2 from FUME-TCRseq Enables Sensitive and Accurate Sequencing of the T-cell Receptor from Limited Input of Degraded RNA

A, Scatter plot showing V gene usage data from FUME-TCRseq and 5′-RACE ligation (“5′-RACE TCRseq”) methodologies, performed on RNA extracted from a representative whole blood sample. B, Bar plot showing the proportion of each V gene detected by FUME-TCRseq and 5′-RACE ligation. Red bars, highly frequent V genes; blue, less frequent V genes. C, Scatterplot showing overlapping clonotype abundances for the FUME-TCRseq and 5′RACE TCRseq data. Point size is scaled to the geometric mean of clonotype frequency in both samples and axes represent log₁₀ clonotype frequencies in each sample. D, Plot showing the 10 most common clonotypes identified by FUME-TCRseq and the frequencies at which they were detected using 5′-RACE ligation methodology. E, Scatterplot showing overlapping clonotype abundances for technical repeats. Point size is scaled to the geometric mean of clonotype frequency in both samples and axes represent log₁₀ clonotype frequencies in each sample. F, Plot showing the 10 most common clonotypes detected in technical repeats of FUME-TCRseq performed on RNA extracted from an inflammatory polyp.