Figure 5 from FUME-TCRseq Enables Sensitive and Accurate Sequencing of the T-cell Receptor from Limited Input of Degraded RNA

A, Bar plots showing the proportion of each V gene detected by FUME-TCRseq (left column) and Immunoverse (right column) in two representative FFPE colorectal cancer samples (PIK3CA WT, top row; PIK3CA E545K, bottom row). B, Bar chart showing the numbers of unique TCR clonotypes detected in the two samples with FUME-TCRseq and Immunoverse methodologies. C, Plot showing the frequencies of the 10 most common clonotypes detected in the WT sample by FUME-TCRseq and the frequencies at which they appear in the Immunoverse data. D, Plot showing the frequencies of the 10 most common clonotypes detected in E545K sample by FUME-TCRseq and the frequencies at which they appear in the Immunoverse data.