A variety of substances and herbal supplements commonly available over- the-counter may affect and interfere with biochemical assays and lead to false results which may lead to unnecessary additional tests and potentially inappropriate treatments. One such substance is biotin, Vitamin B7. Biotin is a very commonly used supplement for healthy hair and nail growth. It is well-known that its use can result in falsely high levels of T4 and T3 and falsely low TSH leading to false diagnosis of hyperthyroidism.
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Khan et al. (2024) studied this question.
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