Mp32-18 Role of Hypogonadism in Urethral Stricture Formation in Rabbit Urethral Stricture Model

You have accessJournal of UrologyReconstruction: Urethral Reconstruction (Including Stricture) II (MP32)1 May 2024MP32-18 ROLE OF HYPOGONADISM IN URETHRAL STRICTURE FORMATION IN RABBIT URETHRAL STRICTURE MODEL Guanqun Li, Maria A. Ortiz, Elizabeth Bearrick, and Dmitriy Nikolavsky Guanqun LiGuanqun Li , Maria A. OrtizMaria A. Ortiz , Elizabeth BearrickElizabeth Bearrick , and Dmitriy NikolavskyDmitriy Nikolavsky View All Author Informationhttps://doi.org/10.1097/01.JU.0001008816.80828.35.18AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract INTRODUCTION AND OBJECTIVE: Animal urethral stricture models are important in studying urethral stricture disease. One of the common ones involves endoscopic urethral mucosa electroresection using male rabbits. Despite technical feasibility, rates of successful stricture formation after the first induction range as low as 18% to 27%. Re-inductions were required in a high proportion of animals. This adds stress to the animals, increases time and cost. Prior studies in human showed that hypogonadism is associated more severe urethral stricture. Our aim was to evaluate the effect of castration on stricture induction, and to create a more efficient stricture induction animal model. We hypothesized that hypogonadal state increases chances of stricture formation after induced urethral mucosal trauma. METHODS: After IACUC and IRB approvals, 10 adult male New Zealand rabbits were randomized into control (5) and treatment group (5). Treatment rabbits underwent bilateral orchiectomy. Two months later, all rabbits underwent stricture induction: a 1cm long longitudinal incision was made in the urethral mucosa using a 10Fr pediatric resectoscope. The incision extended from the 3 to 9 o'clock position. The defect was created in an area of bulbar urethra approximately 1 cm distal to the external sphincter and 2 cm proximal to the meatus. 6 weeks after induction a retrograde urethrogram (RUG) was performed to evaluate for degree of stenosis. Cystoscopy was performed to evaluate cystoscopic evidence of stricture. Our endpoints were radiographic and cystoscopic success in stricture induction. RESULTS: In the castrated group, 5/5 (100%) developed stricture with at least 30% lumen stenosis, with a mean lumen reduction of 55% (36.5%-79.6%). In the control group, 2/5 (40%) developed stricture of at least 30% lumen stenosis, with a mean 27% (20.5%-32.1%), p=0.005. More severe strictures were observed in the castrated group. 3/5 (60%) developed stricture>50%, while none in the control. On cystoscopic evaluation, 4/5 (90%) rabbits in the castrated group developed severe stricture (unable to advance scope through), while none in the control. CONCLUSIONS: Induction of hypogonadism the rabbits prior to stricture induction by electroresection increases stricture induction rate and severity. Further histological study needs to be performed to investigate histological changes. Download PPT Source of Funding: N/A © 2024 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 211Issue 5SMay 2024Page: e521 Advertisement Copyright & Permissions© 2024 by American Urological Association Education and Research, Inc.Metrics Author Information Guanqun Li More articles by this author Maria A. Ortiz More articles by this author Elizabeth Bearrick More articles by this author Dmitriy Nikolavsky More articles by this author Expand All Advertisement PDF downloadLoading ...