MP32-19 PLACENTAL MEMBRANE GRAFTS FOR THE TREATMENT OF URETHRAL STRICTURES IN a RABBIT MODEL

You have accessJournal of UrologyReconstruction: Urethral Reconstruction (Including Stricture) II (MP32)1 May 2024MP32-19 PLACENTAL MEMBRANE GRAFTS FOR THE TREATMENT OF URETHRAL STRICTURES IN a RABBIT MODEL Gregory Mansour, Erik Grossgold, Kamala Rapp-Santos, Kurt McCammon, Jack Zuckerman, Chad Pusateri, Xiaofei Qin, and Davorka Softic Gregory MansourGregory Mansour , Erik GrossgoldErik Grossgold , Kamala Rapp-SantosKamala Rapp-Santos , Kurt McCammonKurt McCammon , Jack ZuckermanJack Zuckerman , Chad PusateriChad Pusateri , Xiaofei QinXiaofei Qin , and Davorka SofticDavorka Softic View All Author Informationhttps://doi.org/10.1097/01.JU.0001008816.80828.35.19AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract INTRODUCTION AND OBJECTIVE: Several graft materials are available for use in the treatment of urethral stricture disease. Placental membrane is being used in a variety of settings as a tissue bridge in wound healing. In our previously published pilot study, we demonstrated urothelial replacement of human placental membrane in the rabbit urethra without stricture formation. We aim to further evaluate the effect of implanting decellularized human placental membrane into rabbit urethras in a larger cohort. We hypothesize that the urethra will remain patent on cystoscopic examination, urothelial replacement of the placental membrane graft will be observed, and use of the placental membrane graft will lead to improved patency and reduction in scar formation. METHODS: Dorsal onlay free graft urethroplasties utilizing harvested and prepared human placental membrane were performed in 27 New Zealand White rabbits (oryctolagus ouniculus). 6 control rabbits underwent a dorsal urethrotomy and urethroplasty without graft implantation. The 27 treatment and 6 control group rabbits were divided into two experimental groups. Arm 1 included 14 treatment/3 controls and was observed postoperatively for 6 months. Arm 2 included 13 treatment/3 controls and was observed postoperatively for 9 months. The rabbits underwent cystourethroscopy to evaluate urethral patency. The rabbits were then euthanized and the urethras were examined pathologically. RESULTS: All urethroplasties were performed without complication. Cystourethroscopy showed no stricture formation in either study group. Both 6 and 9 month treatment groups had improved appearance on urethroscopy. Pathologic examination showed similar degrees of urethral replacement in between treatment and control groups. CONCLUSIONS: Dorsal onlay urethroplasty using decellularized human placental membrane can safely be performed in a rabbit model. Additional studies are needed to validate placental membrane as a potential graft material. Source of Funding: Institutional Funding © 2024 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 211Issue 5SMay 2024Page: e522 Advertisement Copyright & Permissions© 2024 by American Urological Association Education and Research, Inc.Metrics Author Information Gregory Mansour More articles by this author Erik Grossgold More articles by this author Kamala Rapp-Santos More articles by this author Kurt McCammon More articles by this author Jack Zuckerman More articles by this author Chad Pusateri More articles by this author Xiaofei Qin More articles by this author Davorka Softic More articles by this author Expand All Advertisement PDF downloadLoading ...