FIGURE 5 from The Cross-talk Between Intestinal Microbiota and MDSCs Fuels Colitis-associated Cancer Development

Microbiota depletion in CAC-bearing mice reduces colon inflammation and tumor load. A, Schematic presentation of CAC/ABX experiment and fecal sample collection. Created with BioRender.com. B, Weight change of control, CAC, and ABX-treated CAC mice during disease progression normalized to mice weight at the start point (n = 6). Statistical analysis was performed comparing between CAC and ABX-treated CAC mice from day 51 to day 65. C, Representative images of colons. Tumor load is summarized (right; n = 6). D, Representative H&E-stained colon section. Magnification: 10x, Scale bars: 200 µm. E, FITC-dextran fluorescence intensity in serum normalized to control mice (n = 5–6). F, Spleen weights (n = 6). Levels of TNFα (G), IL6 (H), IL1β (I), IL1α (J), and IL10 (K) in supernatants of cultured colon punch biopsies (n = 6–8). Data representing two independent experiments. Results are represented as mean ± SEM; *, P < 0.05; **, P < 0.01; ***, P < 0.001.