FIGURE 6 from The Cross-talk Between Intestinal Microbiota and MDSCs Fuels Colitis-associated Cancer Development

Microbiota depletion in CAC-bearing mice reduces MDSCs levels and their suppressive function. Flow cytometry data showing the percentages of PMN-MDSCs (A) and M-MDSCs (B) in the blood, spleen, BM, and colon (n = 4–6). Cell percentages were calculated from the total cell populations. C, Flow cytometry analysis showing CD247 expression levels in splenic T cells (n = 6), calculated from CD3⁺ cells. Representative data from one of three independent experiments. D, Proliferation of activated T cells that were cocultured with M-MDSCs isolated from spleens of CAC or ABX-treated CAC mice at a ratio of 1:0.25. Representative histograms (left) and the summarized proliferation index (right) are presented (n = 4). E, Expression levels of CD247 in naïve T cells cocultured with PMN-MDSCs isolated from the spleen of CAC and ABX-treated CAC mice at a ratio of 1:1 is depicted. Representative histograms (left) and summarized MFI (right) are shown (n = 4). Data represent two independent experiments. Results are represented as mean ± SEM; *, P < 0.05; **, P < 0.01; ***, P < 0.001.