FIGURE 1 from The Cross-talk Between Intestinal Microbiota and MDSCs Fuels Colitis-associated Cancer Development

CAC-bearing mice display site specific and systemic inflammation during disease progression. A, Schematic presentation of the AOM/DSS-based CAC model used. Created with BioRender.com. B, Weight change during disease progression, normalized to the start point (n = 8 per group). C, Representative images of colons. D, H&E staining of the colon (top) and spleen (bottom) tissue sections of control and CAC mice; Magnification: 10x, Scale bar: 200 µm. E, Spleen weights (n = 8). Levels of TNFα (F), IL6 (G), IL1β (H), and IL10 (I) in supernatants of cultured colon punch biopsies (n = 5 or 10). In J–M, black dots represent the control group and red dots represent the CAC group. Flow cytometry data showing percentages of PMN-MDSCs (J) and M-MDSCs (K) in the blood, spleen, BM, and colon. Cell percentages were calculated from total cell populations. L, Flow cytometry data showing expression levels of CD247 (presented by MFI) in CD3ε+ T cells in blood, spleen, BM, and colon (n = 3–8). M, Flow cytometry data showing expression levels of CD3ε (presented by MFI) in blood, spleen, BM, and colon (n = 3–8). Expression levels of CD247 were calculated from CD3⁺ cells. Representative data from one out of three independent experiments. N, Representative histograms illustrate the proliferation of activated T cells cocultured with different ratios of M-MDSCs isolated from the spleens of CAC mice (left). The corresponding proliferation index is summarized on the right (n = 4). O, Representative histograms of CD247 expression levels in naïve T cells cocultured with different ratios of PMN-MDSCs isolated from spleen of CAC mice (left). The corresponding CD247 levels are summarized as MFI on the right (n = 4). Results are represented as mean ± SEM. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001.