FIGURE 3 from The Cross-talk Between Intestinal Microbiota and MDSCs Fuels Colitis-associated Cancer Development

CAC-associated microbiota promotes tumor development. A, Schematic presentation of CAC/FMT experiment and fecal sample collection. Created with BioRender.com. B, Weight change in CAC-induced mice during disease progression (n = 7–9). Weights are normalized to the starting point. C, Representative images of colons (left) and summarized tumor load (right). Black arrowheads represent macroscopic tumors. D, Representative H&E staining of colon sections. E, Spleen weights. Flow cytometry data showing the percentages of PMN-MDSCs (F) and M-MDSCs (G) in the spleens (n = 7–9). H, PCoA plots showing microbiota composition of control (n = 2) and CAC (n = 3) donor samples (pools) and fecal samples of recipient mice 14 days after microbiota transfer (T1). I, PCoA plots showing microbiota composition analysis of control and CAC-induced control-FMT and CAC-FMT mice at all timepoints (T1–T5) during the course of the experiment. Results are presented as mean ± SEM; *, P < 0.05; **, P < 0.01.