FIGURE 8 from Identification of Enhanced Vaccine Mimotopes for the p15E Murine Cancer Antigen

TCR sequencing of wild-type p15E-specific CD8 T cells sorted from splenocytes of 3C, 3M, and 3C2V immunized mice. A, Percentage of Ag-specific CD8⁺ T cells in all CD8⁺ T cells. B, Proportion of total repertoire occupied by top 30 most abundance clonotypes. The peptide vaccine dose (in µg) used to induce Ag-specific T cells is shown below the graph. C, Length distribution of observed CDR3 amino acid sequences within each sample. Lines represent mean proportion within each group. Total TCR transcripts (D), Gini coefficient (E), number of unique CDR3 clonotypes (F), and clonality estimates (G) of Ag-specific repertoires. H, Differentially abundant clonotypes observed between groups. Datapoints represent individual values for each mouse. Statistical comparisons are determined by Student t test. Error bars show mean ± SD.