FIGURE 2 from Identification of Enhanced Vaccine Mimotopes for the p15E Murine Cancer Antigen

Functional screening of positional peptide microlibraries reveals 3M and 3C substitutions as p15E e-mimotopes. A, Schematic shows the strategy of two-step e-mimotope screening. Mice were vaccinated with peptide position library vaccines (B) or individual peptide vaccines (C) with the position 3 amino acid being replaced, as indicated. Vaccinations occurred on days 0 and day 7, followed by inoculation of 1 × 10⁶ MC38 cells on day 14. Tumor volume on day 19 after tumor inoculation was recorded. The square symbol in C indicates the mouse was euthanized because of tumor ulceration before day 19. Size (D) and PDI (E) of CPQ liposomes with the indicated peptide. F, Binding of peptides to PoP (lacking cobalt) or CoPoP liposomes after incubation at room temperature for 1 hour. Statistical analysis applied using the unpaired t test. ****, P < 0.0001. Error bars show mean + SD for three independent experiments.