Characterization of Rubisco From Various Plant Sources for Efficient Catalytic Activity Against Carbon Sequestration

To combat the tremendous increase in atmospheric carbon dioxide level a wide number of technologies are being explored.The selection of natural and less destructive way would be by using plants as they are the most natural sinks present in Earth that have the ability to sequester carbon dioxide.This is mainly carried out by RuBisco, a protein found in all phototrophic forms as a major enzyme involved in photosynthesis.The present work focuses on isolating RuBisco protein from plant species taking into account one from each C3 and C4 plants.This was done in order to determine the effective plant with high carbon dioxide sequestering property.The experimental study was done both in normal and carbon dioxide stressed conditions.Rubisco was isolated from tomato and sugarcane plant and it was characterized by SDS PAGE, FT-IR and HPLC technique.To determine the carbon capture by the plant protein CHNSO analysis was carried out.The plant that absorbed higher concentration of carbon was can be identified.