FIGURE 2 from Identification of Nonfunctional Alternatively Spliced Isoforms of STING in Human Acute Myeloid Leukemia

Evaluation of the STING pathway across common AML cell lines. A, Indicated AML cell lines plated 1 × 10⁵ cells/well were stimulated 72 hours with CDNs at 10 µg/mL, and supernatants were harvested for ELISA (n = 5). An assay detection limit of 50 pg/mL is shown. qPCR analysis (B) and immunoblot (C) of STING pathway and resistance genes across AML lines (n = 1). For qPCR, data represent GAPDH-normalized fold expression relative to normal CD11b⁺ PBMC controls.