Figure S5 from Genetic Silencing of AKT Induces Melanoma Cell Death via mTOR Suppression

Supplemental Figure 5 shows that caspase 3 and 8 inhibition protects melanoma cells from siAKT1-3-mediated apoptosis. A, Cell confluency assay upon treatment with siAKT1-3 versus co-treatment with caspase inhibitors. Co-treatment of A375 and MTG004 cells with Z-VAD-FMK, Z-DEVD-FMK, and Z-IETD-FMK inhibitors protected cells against siAKT1-3-mediated cell death. B, Incuycte® Cytotox Red assay comparing siCtrl, siAKT1-3, siCtrl + each caspase inhibitor, and siAKT1-3 + caspase inhibitors. C, Incuycte® Caspase 3/7 assay comparing siCtrl, siAKT1-3, siCtrl + each caspase inhibitor, and siAKT1-3 + caspase inhibitors. The percentage of dead cells stained with Cytotox red and caspase 3/7 reagents increased only in the siAKT1-3 group, indicating complete rescue with caspase 3, 8, or pan inhibition.