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March 4, 2024Open Access

Figure S4 from Genetic Silencing of AKT Induces Melanoma Cell Death via mTOR Suppression

Gennie L. Parkman Tursun Turapov David A. Kircher William J. Burnett Christopher M. Stehn Kayla O’Toole Katie M. Culver Ashley T. Chadwick Riley C. Elmer Ryan Flaherty Karly A. Stanley Mona Foth David H. Lum Robert L. Judson‐Torres John E. Friend Matthew W. VanBrocklin Martin McMahon Sheri L. Holmen

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Abstract

Supplementary Figure S4 demonstrates that siAKT1-3 is specific for human AKT. A, The cell confluency assay of YUMM1.1 mouse melanoma cells under genetic inhibition demonstrated no effect on cell death with siAKT1, siAKT2, siAKT3, or any combination. Error bars indicate standard error of the mean of triplicate wells. B, Immunoblotting of YUMM1.1 cells treated with siCtrl vs siAKT1-3. No knockdown of P-AKT (Ser473) or P-PRAS40 (T246) indicated the specificity of the siRNAs for human AKT.

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Cite This Study

Parkman et al. (2024) studied this question.

synapsesocial.com/papers/68e75dd0b6db6435876d4cbf https://doi.org/10.1158/1535-7163.25337885.v1

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Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

1Figure S3 from Genetic Silencing of AKT Induces Melanoma Cell Death via mTOR Suppression2024
2Figure S5 from Genetic Silencing of AKT Induces Melanoma Cell Death via mTOR Suppression2024
3Figure S1 from Genetic Silencing of AKT Induces Melanoma Cell Death via mTOR Suppression2024
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