RP-HPLC Method Development and Validation of the Simultaneous Estimation of Glecaprevir and Pibrentasvr in Bulk and Dosage Forms

A Simple, Accurate, Precise method was developed for the simultaneous estimation of the Glecaprevir and Pibrentasvir in Tablet dosage form.Chromatogram was run through Inertsil C18 150 x 4.6 mm, 5m.Mobile phase containing Buffer 0.01N KH2PO4: Acetonitrile taken in the ratio 60:40 was pumped through the column at a flow rate of 1 ml/min.The buffer used in this method was 0.01 NKH2PO4 buffer.The optimized wavelength selected was 260 nm.The retention time of Glecaprevir and Pibrentasvir was found to be 2.509 min and 3.111 min.%RSD of the Glecaprevir and Pibrentasvir were found to be 0.2 and 0.6 respectively.%Recovery was obtained as 99.47% and 99.57% for Glecaprevir and Pibrentasvir respectively.LOD and LOQ values of Glecaprevir and Pibrentasvir were 0.36, 1.0,8, and 0.16, 0.48 respectively.Retention times were decreased, so the method developed was simple and economical and can be adopted in regular Quality control tests in Industries.