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October 9, 2025Circulation Research

Abstract Wed120: Right Ventricle Fibroblasts Display a Hyperfibrotic Phenotype In Vitro

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Key result

RV fibroblasts show ~2-fold greater proliferation and higher periostin expression than LV fibroblasts.

  • P<0.05

Why the study?

Do right ventricular fibroblasts display a distinct, hyperfibrotic phenotype compared to left ventricular fibroblasts in a rat model of pulmonary hypertension?

Population

Sugen-Hypoxia rat model and isolated right and left ventricular fibroblasts

Comparison

Isolation and in vitro analysis of right… vs Left ventricular fibroblasts and baseline RVfibs

Design

Preclinical

Follow-up

up to 6 weeks post-hypoxia in vivo; 3 days in vitro

Authors

MBMadeline BurghazeThe Ohio State UniversityMGMatthew W. GorrThe Ohio State University Wexner Medical Center

Discussion

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Implication

Right ventricular fibroblasts exhibit an intrinsically amplified proliferative and fibrotic response compared to left ventricular fibroblasts, which may explain the severe RV fibrosis seen in pulmonary hypertension.

Key Points

  • Right ventricle fibroblasts show significantly higher fibrotic gene expression compared to left ventricle fibroblasts, indicating a unique response to stress.
  • Quantitative PCR analysis revealed a 1.2 times higher fold-change in periostin expression in right ventricle fibroblasts stimulated by TGFβ than their left ventricle counterparts.
  • Seahorse assay results suggest that right ventricle fibroblasts have a greater oxygen consumption rate than left ventricle fibroblasts, highlighting their metabolic differences.
  • RNA-seq analyses indicate that right ventricle fibroblasts favor pathways linked to cell division, potentially driving their hyperfibrotic phenotype.

Structured PICO

Do right ventricular fibroblasts display a distinct, hyperfibrotic phenotype compared to left ventricular fibroblasts in a rat model of pulmonary hypertension?

P
Population
In vitro and animal model study using the Sugen-Hypoxia rat model to characterize right versus left ventricular fibroblasts in pulmonary hypertension.
E
Exposure
Isolation and in vitro analysis of right ventricular fibroblasts (RVfibs), including stimulation with 10 ng/mL TGFβ
C
Comparator
Left ventricular fibroblasts (LVfibs) and baseline RVfibs
O
Outcome
Fibrotic gene expression (periostin), proliferation rate, and oxygen consumption rate (OCR)surrogate

Main Result

p-value: p=<0.05

Right ventricular fibroblasts exhibit an intrinsically amplified proliferative and fibrotic response compared to left ventricular fibroblasts, which may explain the severe RV fibrosis seen in pulmonary hypertension.

Cite This Study

Burghaze et al. (2025) studied Pulmonary hypertension. Right ventricular fibroblasts vs. Left ventricular fibroblasts was evaluated on Periostin expression fold-change and proliferation rate (p=<0.05). Right ventricular fibroblasts exhibited a 1.2 times higher fold-change in periostin expression and twice the proliferation rate compared to left ventricular fibroblasts (p<0.05).

synapsesocial.com/papers/68e77f09d1c187e1c108fbffhttps://doi.org/10.1161/res.137.suppl_1.wed120
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1A64-02 Latent Transcriptional Programs Reveal Progressive Right Ventricular Fibroblast Remodeling During Hypoxia Induced Pulmonary Hypertension2026
  2. 2A107-12 Mitochondrial Oxidative Phosphorylation Critically Regulates Right Ventricular Fibroblast Function in Pulmonary Hypertension2026
  3. 3Right Ventricular Fibrosis2019 · 199 citations
  4. 4Fibroblasts and the extracellular matrix in right ventricular disease2017 · 92 citations
  5. 5Modulators of right ventricular apoptosis and contractility in a rat model of pulmonary hypertension2016 · 56 citations