SMART v10: three decades of the protein domain annotation resource

SMART (Simple Modular Architecture Research Tool, https://smart.embl.de) is a web-based platform for identifying and annotating protein domains and analyzing domain architectures. SMART version 10 features manually curated models for over 1300 protein domains. Approaching its 30th anniversary, SMART’s user interface has been redesigned from the ground up, leveraging modern web technologies to enhance intuitiveness and usability. SMART’s “Genomic” mode, which annotates proteins from completely sequenced genomes was synchronized with the current release of STRING, and now includes 12 035 species, compared to 5090 in the previous release. Protein and domain annotation pages have been updated with new information sources. Integration with eggNOG provides links to 17.5 million orthologous groups for over 53 million proteins. Additionally, synchronization with the interactive Pathways Explorer version 3 incorporates updated KEGG pathway and orthologous group data, enabling direct visualization on four distinct pathway overview maps.