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October 18, 2025CancersOpen Access

CRISPR with a Double Mismatch Guide RNA Enhances Detection Sensitivity for Low-Frequency Single-Base EGFR Mutation in Circulating Cell-Free DNA of Lung Cancer Patients

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Authors

KBKyung Wook BeenSKSeunghun KangTBTaegeun Bae

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Overview

This study demonstrates that a CRISPR-based diagnostic system enhances mutation detection sensitivity in circulating DNA, suggesting a novel approach for lung cancer diagnostics.

Key Points

  • The CRISPR/Cas12a-based system effectively detects low-frequency EGFR mutations in circulating DNA from lung cancer patients.
  • All 7 L858R-positive samples and 6 out of 11 exon 19 deletion samples were successfully identified, indicating strong diagnostic capability.
  • The approach involved selectively cleaving WT DNA to enrich mutant sequences, facilitating better mutation identification.
  • This liquid biopsy technique highlights the potential for non-invasive detection of oncogenic mutations in various cancers.

Cite This Study

Been et al. (2025) studied this question.

synapsesocial.com/papers/68f396388da44caaba02c6f4https://doi.org/10.3390/cancers17203343
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1CRISPR-BASED APPROACHES FOR THE DETECTION AND TARGETING OF LUNG CANCER BIOMARKERS: A NEW ERA IN PRECISION ONCOLOGY2025
  2. 2CRISPR/Cas13a-based supersensitive circulating tumor DNA assay for detecting EGFR mutations in plasma2024 · 23 citations
  3. 3A “turn-on” CRISPR-mediated method using enhanced fluorescent bimetallic DNA nanoclusters for EGFR mutation detection in non-small cell lung cancer2026
  4. 4An ultrasensitive DNA-enhanced amplification method for detecting cfDNA drug-resistant mutations in non-small cell lung cancer with selective FEN-assisted degradation of dominant somatic fragments2024
  5. 5Liquid Biopsy for EGFR Mutation Detection in NSCLC: Evaluation of Plasma ctDNA and Comparison with Plasma exoDNA2025