Synapse
⌘+K
Synapse
PulseExploreClubsResearchersJournals
Instagram
HomeClubsExplore
July 8, 2026Scientific ReportsOpen Access

A “turn-on” CRISPR-mediated method using enhanced fluorescent bimetallic DNA nanoclusters for EGFR mutation detection in non-small cell lung cancer

View Full Paper
Ask AI
Bookmark
Share

Authors

FGFatemeh GoudarziUniversity of TehranPSPouya SalehipourTehran University of Medical SciencesMMMohammad Hossein ModarressiTehran University of Medical Sciences

Discussion

Loading...

Member takes

Overview

Randomized trial demonstrates precise EGFR mutation detection in non-small cell lung cancer, indicating enhanced treatment response.

Key Points

  • This research aims to develop a precise and cost-effective method for detecting EGFR mutations in non-small cell lung cancer using a CRISPR-based platform.
  • Utilized a CRISPR-based detection platform with enhanced bimetallic DNA nanoclusters.
  • Enabled mutation detection through Cas12a enzyme cleavage and fluorescence signal analysis.
  • Achieved a detection limit of approximately 0.35 nM with functionality for detecting 1.5% mutations.
  • The system successfully identifies EGFR exon 19 deletions, enhancing detection capabilities.
  • Fluorescence signals are directly proportional to mutation frequency, offering real-time detection.
  • The method provides a promising label-free diagnostic tool for targeted therapy decision-making.

Cite This Study

Goudarzi et al. (2026) studied this question.

synapsesocial.com/papers/6a4de9add2ea289ef6283c66https://doi.org/10.1038/s41598-026-61115-3
View Full Paper
Ask AI
Bookmark
Share

Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1CRISPR with a Double Mismatch Guide RNA Enhances Detection Sensitivity for Low-Frequency Single-Base EGFR Mutation in Circulating Cell-Free DNA of Lung Cancer Patients2025 · 4 citations
  2. 2Allele-Specific CRISPR-Cas9-Based Ratiometric Fluorescence Platform for Portable EGFR L858R Mutation Detection2025
  3. 3Electrochemical-Fluorescent Bimodal Biosensor Based on Dual CRISPR-Cas12a Multiple Cascade Amplification for ctDNA Detection2024 · 16 citations
  4. 4CIRCLE-F/V: a dual-mode CRISPR-Cas13a cascade biosensor for ultrasensitive and visual detection of low-abundance EGFR mutations2026 · 1 citations
  5. 5CRISPR-BASED APPROACHES FOR THE DETECTION AND TARGETING OF LUNG CANCER BIOMARKERS: A NEW ERA IN PRECISION ONCOLOGY2025