Synapse
⌘+K
Synapse
PulseExploreClubsResearchersJournals
Instagram
HomeClubsExplore
March 29, 2026Journal of Biological EngineeringOpen Access

CIRCLE-F/V: a dual-mode CRISPR-Cas13a cascade biosensor for ultrasensitive and visual detection of low-abundance EGFR mutations

View Full Paper
Ask AI
Bookmark
Share

Authors

TZTao ZhuNingbo UniversityZWZixuan WangNingbo UniversityYWYujia WuNingbo University

Discussion

Loading...

Member takes

Implication

CIRCLE-F/V demonstrates ultrasensitive detection of EGFR mutations in non-small cell lung cancer, indicating its clinical potential.

Key Points

  • The study aims to develop a biosensor for sensitive detection of low-frequency EGFR mutations in NSCLC.
  • Integrated restriction-assisted mutant enrichment with cyclic CRISPR signal amplification.
  • Genomic DNA digested with MscI to remove wild-type alleles.
  • T7 promoter-mediated PCR amplification and in vitro transcription for RNA production.
  • Activation of Cas13a-crRNA complexes for signal generation.
  • Visual readout achieved through lateral flow strips with a biotin probe.
  • Fluorescence mode sensitivity achieved a variant allele frequency of 0.01%.
  • Genomic DNA detection limit estimated at ~3.6 fM.
  • RNA detection limit reached ~2.16 pM under optimized conditions.
  • Complete concordance with next-generation sequencing for mutation classification.

Cite This Study

Zhu et al. (2026) studied this question.

synapsesocial.com/papers/69c8c371de0f0f753b39e2f2https://doi.org/10.1186/s13036-026-00675-4
View Full Paper
Ask AI
Bookmark
Share

Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1CRISPR/Cas13a-based supersensitive circulating tumor DNA assay for detecting EGFR mutations in plasma2024 · 25 citations
  2. 2Lung cancer mortality and associated predictors: systematic review using 32 scientific research findings2023 · 28 citations
  3. 3CRISPR-Cas12a target binding unleashes indiscriminate single-stranded DNase activity2018 · 4,654 citations
  4. 4Activating Mutations in the Epidermal Growth Factor Receptor Underlying Responsiveness of Non–Small-Cell Lung Cancer to Gefitinib2004 · 11,596 citations
  5. 5CRISPR-Cas13a system: a novel approach to precision oncology2020 · 16 citations