What question did this study set out to answer?

To develop a protein scaffold targeting the transferrin receptor for drug delivery and antiviral applications.

November 18, 2025Protein ScienceOpen Access

Targeting the apical domain of the transferrin receptor: Development of a new protein scaffold for cellular delivery

View Full Paper

Ask AI

Bookmark

Authors

Development of a novel protein scaffold enhances drug delivery and targets pathogens in cells, suggesting new antiviral options.

To develop a protein scaffold targeting the transferrin receptor for drug delivery and antiviral applications.
Designed a small protein scaffold through computational docking to the transferrin receptor apical domain.
Expressed candidate proteins using yeast surface display and evaluated interactions via flow cytometry.
Optimized a variant using directed evolution for improved binding to transferrin receptor.
One optimized construct showed nanomolar binding affinity to the transferrin receptor.
The evolved protein demonstrated cellular uptake comparable to FITC-coupled transferrin.
The scaffold can be utilized for targeted drug delivery and development of antiviral therapeutics against arenaviruses.

Veetil et al. (2025) studied this question.

View Full Paper

Ask AI

Bookmark