What question did this study set out to answer?

To evaluate the long-term effects of biochar on soil properties and microbial communities.

November 15, 2025Microbiological ResearchOpen Access

Eight-year effect of biochar amendment on soil properties, extracellular enzyme activity, N-cycling genes and microbiome structure in two Danish fallow soils

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Field study reveals biochar enhances soil properties and microbial diversity over 8 years, indicating lasting benefits.

To evaluate the long-term effects of biochar on soil properties and microbial communities.
Field-aged biochar was applied to the topsoil of two soils: sandy clay and coarse sandy.
Microbiomes were assessed for changes in biodiversity and soil properties over an 8-year period.
Extracellular enzyme activity and N-cycling gene abundance were measured at the end of the study.
Biochar significantly improved soil physicochemical properties and enhanced microbial diversity.
Extracellular enzyme activity increased significantly, including α-glucosidase and β-galactosidase.
Key nitrification and denitrification genes showed increased abundance, indicating sustained N cycling.

Espinoza et al. (2025) studied this question.

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