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This research aims to explore the long-term impacts of aged biochar on soil nitrogen dynamics and microbial influences during growth stages.

Biochar‐Induced Shifts in Soil Nitrogen Fractions and Microbial Nitrogen Demand After a Decade of Conservation Tillage

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An 11-year experiment demonstrates biochar's impact on soil nitrogen in a winter wheat-maize rotation, highlighting its ecological significance.

This research aims to explore the long-term impacts of aged biochar on soil nitrogen dynamics and microbial influences during growth stages.
Conducted an 11-year field experiment with varying biochar application rates (0, 30, 60, 90 Mg ha −1 )
Measured total native nitrogen, soil organic nitrogen fractions, total dissolved nitrogen, and gross nitrogen mineralisation in calcareous soil
Analyzed nitrogen dynamics during winter wheat-maize rotation
Biochar application increased total native nitrogen by 7%-8% and recalcitrant soil organic nitrogen by 52%-104% in deeper soil layers.
Labile soil organic nitrogen pools increased with biochar, while less labile pools decreased overall compared to unamended soil.
During crop growth, biochar-amended soil exhibited increased total dissolved nitrogen and gross nitrogen mineralisation in the top 15 cm.

Ding et al. (2026) studied this question.

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An 11-year experiment demonstrates biochar's impact on soil nitrogen in a winter wheat-maize rotation, highlighting its ecological significance.