Synthesis of β‐Cyclodextrin–Calix[4]arene Polymer‐Based Chiral Stationary Phase for Chromatographic Separation of Enantiomers

In this work, a new strategy was proposed for the preparation of a chiral stationary phase based on β‐cyclodextrin and calix[4]arene polymer. The chiral stationary phase was characterized by scanning electron microscopy, energy dispersive x‐ray spectroscopy, Fourier transform infrared spectroscopy, and thermogravimetric analysis. The introduction of calix[4]arene polymer increased chirality recognition by increasing recognition sites and interactions, including hydrogen bonding, π–π interaction and synergistic inclusion effect. Compared with the home‐made β‐cyclodextrin–based chiral column, the prepared β‐cyclodextrin–calix[4]arene polymer‐based chiral column showed superior enantioseparation performance toward 1‐phenylethanol, prothioconazole, promethazine, propranolol, and bisoprolol. These chiral compounds can be separated within 10 min under reversed‐phase mode with resolutions ranging from 1.62 to 1.80. These findings will provide an important reference for developing novel supramolecule‐based chiral stationary phases.