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December 8, 2025BloodOpen Access

Correlation between CRLF2 expression by flow cytometry and FISH in patients diagnosed with B-cell acute lymphoblastic leukemia

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Authors

FSFlávia SousaUniversity of FribourgBNBruna Garcia NogueiraHospital Israelita Albert EinsteinRSRoberta SafranauskasHospital Israelita Albert Einstein

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Implication

Retrospective analysis shows CRLF2 gene expression via flow cytometry aligns with FISH in B-ALL, indicating effective monitoring.

Key Points

  • This study investigates the correlation between CRLF2 expression assessed by flow cytometry and FISH in B-ALL patients.
  • Retrospective analysis of 39 diagnostic samples from B-ALL patients
  • Assessment of CRLF2 expression by flow cytometry using a specific panel of monoclonal antibodies
  • Comparison of CRLF2 expression results with FISH analysis for genetic confirmation
  • 14.3% of patients showed CRLF2 overexpression by flow cytometry with confirmed rearrangements by FISH
  • 100% concordance between flow cytometry and FISH at diagnosis
  • Flow cytometry demonstrated superior sensitivity for detecting measurable residual disease (MRD) compared to FISH

Cite This Study

Sousa et al. (2025) studied this question.

synapsesocial.com/papers/69362f364fa91c937236d2c6https://doi.org/10.1182/blood-2025-6832
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Genetic characteristics and treatment outcomes of pediatric acute lymphoblastic leukemia patients with CRLF2 rearrangements2025
  2. 2Pediatric B-lineage acute lymphoblastic leukemia with CRLF2 rearrangements. Own experience in molecular genetic diagnostics and patient monitoring2025
  3. 3Pediatric B-lineage acute lymphoblastic leukemia with CRLF2 rearrangements. Own experience in molecular genetic diagnostics and patient monitoring2025
  4. 4Combination of p-STAT5, CRLF2 and copy number alterations as a potential indicator of a high-risk subgroup of acute lymphoblastic leukemia2026
  5. 5Outcomes of concomitant Philadelphia chromosome-positive and CRLF2-rearranged B-cell ALL2025