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December 8, 2025BloodOpen Access

Phenotyping of ex vivo expanded NK cells reveals unique transcriptomic and epigenetic signatures

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Authors

RRRuyan RahnamaJohns Hopkins UniversityCBChallice L. BonifantJohns Hopkins University

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Overview

Research identifies unique gene expression and DNA methylation patterns in expanded NK cells, indicating improved immunotherapy potentials.

Key Points

  • This research aims to understand the unique transcriptomic and epigenetic signatures of ex vivo expanded NK cells for potential cancer immunotherapy applications.
  • Depleted CD3+ cells from human peripheral blood mononuclear cells
  • Cultured CD3- cells with irradiated K562.mbIL15.41BBL or K562.mbIL21.41BBL cells and various cytokines
  • Analyzed cell products using flow cytometry, bulk RNA sequencing, and whole genome bisulfite sequencing
  • No differences in NK cell purity or expansion were noted across methods
  • Expanded NK cells exhibited higher expression of activation markers and unique transcriptional profiles
  • Distinct methylation patterns associated with immune regulatory genes were observed in different NK cell expansion conditions

Cite This Study

Rahnama et al. (2025) studied this question.

synapsesocial.com/papers/69362f3d4fa91c937236d47ehttps://doi.org/10.1182/blood-2025-2360
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Single-Cell Transcriptomics Reveals Dynamics of NK Cell Expansion in a Feeder Cell-Free Culture of PBMCs - Implications for Immunotherapy2025
  2. 2Donor Variability and Seeding Density Shape NK-Cell Proliferation and Surface Receptor Expression: Insights from an Integrated Phenotypic and Genetic Analysis2025 · 5 citations
  3. 3More than IL-2: Optimizing feeder-free NK cell function through cytokine synergy2025
  4. 4Human NK Cell Enrichment and Expansion Using Defined GMP Reagents: Functional and Phenotypic Outcomes 22529952026
  5. 5Cytokine-induced expandable memory NK cells with significant metabolic, epigenetic remodeling, and persistence properties2026