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December 8, 2025BloodOpen Access

Tryptophan metabolism plays a significant role in multiple myeloma survival

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Authors

JRJulia Grace ReinkeKCKanita ChaudhryPPPeng Peng

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Overview

Experiment demonstrates kynurenine production affects progression-free survival in multiple myeloma, suggesting metabolic pathways are critical for cell survival.

Key Points

  • To investigate the role of tryptophan metabolism in multiple myeloma survival and its independence from the bone marrow microenvironment.
  • Patient RNA expression data were analyzed from the CoMMpass database.
  • Western blot and qPCR measured tryptophan metabolizing enzyme expression in multiple myeloma cell lines.
  • Tryptophan 2,3-Dioxygenase was inhibited or knocked down to assess its effects on cell survival and KYN production.
  • AHR activation was evaluated using qPCR for CYP1a1 expression after treatment with a TDO inhibitor.
  • Higher TDO expression correlated with significantly lower progression-free survival in patients.
  • Inhibition of TDO reduced survival and KYN production in TDO+ multiple myeloma cell lines.
  • Co-culture with Dendritic Cells or AHR ligand treatment rescued cell viability from TDO inhibitor effects.

Cite This Study

Reinke et al. (2025) studied this question.

synapsesocial.com/papers/69362f484fa91c937236d674https://doi.org/10.1182/blood-2025-5726
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Also Consider

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  5. 5Revisiting tryptophan metabolism in cancer: complexity, context, and spatial heterogeneity2025 · 2 citations