What type of study is this?

This is a In Vitro Study study.

What question did this study set out to answer?

This research aims to uncover the molecular identities and clonal dynamics of measurable residual disease (MRD) in myelodysplastic syndromes (MDS) following stem cell transplant.

December 8, 2025Blood

Single-cell multiomics reveals molecular identities and clonal dynamics of MRD progression in Myelodysplastic Syndromes after stem cell transplant

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Authors

JLJae‐Hyun Lee WLWesley S. Lu AVAlejandro Marinos Velarde

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Overview

Single-cell technology reveals MRD progression dynamics in myelodysplastic syndromes post stem cell transplant, suggesting novel targets for intervention.

Key Points

This research aims to uncover the molecular identities and clonal dynamics of measurable residual disease (MRD) in myelodysplastic syndromes (MDS) following stem cell transplant.
Developed a novel single-cell multi-omic workflow called CARAMEL-seq.
Analyzed 52 longitudinal bone marrow samples from 10 patients who relapsed and 3 who did not.
Profiled both malignant and immune compartments by sorting CD34⁺ and CD34⁻ bone marrow mononuclear cells.
Utilized clonal tracing methods integrating copy number variant data and mitochondrial variant probabilities.
CARAMEL-seq achieved near 10⁻⁷ sensitivity for MRD detection, identifying MRD cells in 9 of 10 relapsed patients.
Observed specific enrichment of MRD cells in the CD34⁺ compartment and identified novel markers of MRD.
Noted significant phenotypic and clonal evolution in post-SCT relapse, with dominant clones being selectively eliminated.
Found increased expression of TGF-β, hypoxia, and IFNγ pathways in relapsed MRD cells.

Cite This Study

Lee et al. (2025) studied this question.

synapsesocial.com/papers/69362f4e4fa91c937236d8c1 https://doi.org/10.1182/blood-2025-674

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December 8, 2025Blood

Single-cell multiomics reveals molecular identities and clonal dynamics of MRD progression in Myelodysplastic Syndromes after stem cell transplant

View Full Paper

Ask AI

Bookmark

Authors

JLJae‐Hyun Lee WLWesley S. Lu AVAlejandro Marinos Velarde

Discussion

Member takes

Overview

Single-cell technology reveals MRD progression dynamics in myelodysplastic syndromes post stem cell transplant, suggesting novel targets for intervention.

Key Points

This research aims to uncover the molecular identities and clonal dynamics of measurable residual disease (MRD) in myelodysplastic syndromes (MDS) following stem cell transplant.
Developed a novel single-cell multi-omic workflow called CARAMEL-seq.
Analyzed 52 longitudinal bone marrow samples from 10 patients who relapsed and 3 who did not.
Profiled both malignant and immune compartments by sorting CD34⁺ and CD34⁻ bone marrow mononuclear cells.
Utilized clonal tracing methods integrating copy number variant data and mitochondrial variant probabilities.
CARAMEL-seq achieved near 10⁻⁷ sensitivity for MRD detection, identifying MRD cells in 9 of 10 relapsed patients.
Observed specific enrichment of MRD cells in the CD34⁺ compartment and identified novel markers of MRD.
Noted significant phenotypic and clonal evolution in post-SCT relapse, with dominant clones being selectively eliminated.
Found increased expression of TGF-β, hypoxia, and IFNγ pathways in relapsed MRD cells.

Cite This Study

Lee et al. (2025) studied this question.

synapsesocial.com/papers/69362f4e4fa91c937236d8c1 https://doi.org/10.1182/blood-2025-674

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