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December 8, 2025BloodOpen Access

Intratumoral B cell diversity in follicular lymphoma revealed by single-cell paired transcriptome and BCR sequencing

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Authors

JWJanek S. WalkerZYZhi-Zhang YangMMMatthew J. Maurer

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Overview

Single-cell analysis reveals diverse B cell populations in follicular lymphoma, suggesting complex tumor biology.

Key Points

  • This research aims to characterize the diversity of B cells within follicular lymphoma tumors using advanced single-cell techniques.
  • Performed single-cell RNA sequencing and BCR sequencing on fresh-frozen lymph node biopsies from 7 FL patients.
  • Classified B cells into expanded and non-expanded populations using transcriptomic and BCR data.
  • Conducted differential expression analysis to compare gene expression between B cell populations.
  • Identified substantial variation in the proportion of ITB cells across tumors, with some tumors containing up to 89% ITB cells.
  • Detected distinct expression patterns and gene usage in different malignant B cell clones within individual tumors.
  • Highlighted significant transcriptional divergence between malignant clones and ITB cells, indicating functional complexity.

Cite This Study

Walker et al. (2025) studied this question.

synapsesocial.com/papers/69362f5a4fa91c937236db38https://doi.org/10.1182/blood-2025-5342
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Abstract A042: Genetic and microenvironmental determinants of site-to-site heterogeneity in follicular lymphoma2026
  2. 2Longitudinal single-cell profiling of the bone marrow heterogeneity identifies the T-cell niche supporting cancer persister cells in follicular lymphoma2025
  3. 3Follicular lymphoma B cells exhibit heterogeneous transcriptional states with associated somatic alterations and tumor microenvironments2024 · 11 citations
  4. 4Integrated Single-Cell and Spatial Multi-Omics of Clonal Precursors and Immune Niches in Germinal Center Lymphomas2026 · 1 citations
  5. 5Integrated single-cell and spatial analysis identifies T cell exhaustion and adhesion signatures in early follicular lymphoma transformation2025