What type of study is this?

This is a Quantitative Study study (also classified as: Validation Study).

What question did this study set out to answer?

To explore imaging flow cytometry as a novel method for assessing splenic function by detecting red blood cell inclusions in sickle cell disease.

December 8, 2025Blood

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Imaging flow cytometry detects RBC inclusions in sickle cell disease, indicating splenic dysfunction and correlation with fetal hemoglobin.

To explore imaging flow cytometry as a novel method for assessing splenic function by detecting red blood cell inclusions in sickle cell disease.
Utilized imaging flow cytometry to identify and quantify inclusions in RBCs from whole blood samples.
Optimized staining protocols using minimal blood volume and selective identification of inclusions without WBC interference.
Analyzed samples from healthy controls and sickle cell disease patients, including those on hydroxyurea therapy and with a history of splenectomy.
In healthy controls, inclusions were rare; in SCD patients with low HbF levels, inclusion frequencies were significantly higher.
HbF levels were negatively correlated with both types of inclusions, suggesting a protective effect on splenic function.
The method provided strong correlations between detected inclusions and manual pitted RBC counts, indicating its reliability.

Hanson et al. (2025) studied this question.

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Imaging flow cytometry detects RBC inclusions in sickle cell disease, indicating splenic dysfunction and correlation with fetal hemoglobin.