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January 14, 2026Open Forum Infectious DiseasesOpen Access

P-1281. Genomic and Transcriptional Adaptations in a Spontaneous Cefiderocol-Resistant Klebsiella pneumoniae KPC-Producing Mutant Isolate

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Authors

ILIrene LuuVMVyanka MezcordJEJenny Escalante

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Overview

Whole-genome sequencing identifies mutations in Klebsiella pneumoniae, revealing alterations in resistance mechanisms and biofilm formation.

Key Points

  • This research examines the genetic basis of cefiderocol resistance in Klebsiella pneumoniae.
  • Whole-genome sequencing was conducted to find mutations linked to cefiderocol resistance.
  • qRT-PCR analyzed gene expression related to iron acquisition and antibiotic resistance.
  • Antimicrobial susceptibility testing determined minimum inhibitory concentrations for various antibiotics.
  • Biofilm formation was quantified using crystal violet assays.
  • IHC216 showed increased cefiderocol MIC, indicating resistance development.
  • Meropenem and imipenem MICs decreased, showing collateral susceptibility.
  • Mutations in genes related to transcriptional regulation and membrane permeability were identified.
  • Key iron acquisition gene expression was downregulated, while alternative pathways were upregulated.

Cite This Study

Luu et al. (2026) studied this question.

synapsesocial.com/papers/6966e73f13bf7a6f02bffec5https://doi.org/10.1093/ofid/ofaf695.1471
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Spontaneous Emergence of Cefiderocol Resistance in Klebsiella pneumoniae KPC-163: Genomic and Transcriptomic Insights2025
  2. 2Mechanistic and evolutionary determinants of cefiderocol resistance in KPC-producing Pseudomonas aeruginosa2026
  3. 3Occurrence of KPC-71 or the presence of NDM combined with a high expression of 2025
  4. 4Exploring mutational possibilities of KPC variants to reach high level resistance to cefiderocol2025 · 10 citations
  5. 5630. Protective Role of Outer Membrane Vesicles Released by Carbapenemase-Producing Klebsiella pneumoniae Clinical Isolates Against Carbapenems and Cefiderocol2026