Rapid Analysis of Rilpivirine, Emtricitabine and Tenofovir Active Ingredients by Novel HPLC Method

Objective: A novel high-performance liquid chromatography (HPLC) method was developed for the simultaneous determination of Emtricitabine (EMT), Tenofovir (TNF), and Rilpivirine (RLP). Material and Method: The analysis was performed using a Waters XBridge C18 (3.5 µm, 4.6 × 150 mm) column. The mobile phase consisted of methanol and triethylamine buffer (pH 3.15) in a 60:40 (v/v) ratio, with a flow rate of 1.2 ml/min. The developed method was successfully applied to standard mixtures and pharmaceutical tablet formulations containing EMT, TNF, and RLP, without matrix interference. Result and Discussion: The proposed technique exhibited good linearity, with correlation coefficients (R²) of 0.999 for EMT, 0.999 for TNF, and 1.000 for RLP. The calibration ranges were 10.0–600.0 µg/ml for EMT, 2.0–75.0 µg/ml for TNF, and 2.0–75.0 µg/ml for RLP. The method was validated in accordance with ICH guidelines, demonstrating high sensitivity, precision, and accuracy. It was found to be a rapid, economical, and efficient approach for the simultaneous determination of these active pharmaceutical ingredients.