ABSTRACT In this study, a novel series of thiazolyl hydrazine derivatives ( T1‐T10 ) were synthesized, and the structures of the obtained compounds were elucidated by 1 H NMR, 13 C NMR, UV, mass, and IR. The compounds were investigated for their in vitro antifungal and antibacterial potentials. Antibacterial assays revealed moderate antibacterial activity across five bacterial strains compared to azithromycin (MIC <0.97 µg/mL for all tested bacteria), with the strongest effects observed against Gram‐positive E. faecalis , where compounds T5 and T6 demonstrated the lowest MIC values (7.81 µg/mL). Moderate activity was also noted for compounds T9 and T10 (15.625 µg/mL). Among Gram‐negative strains, S. marcescens showed susceptibility to compounds T5 and T9 (62.5 µg/mL), while K. pneumoniae remained largely resistant. Antifungal assessments against C. krusei and C. parapsilopsis revealed moderate activity, with compounds T4 , T6 , and T9 exhibiting MIC values of 62.5 µg/mL. The relatively improved activity of these derivatives may be attributed to the presence of halogen substituents and hydrophilic functional groups at specific positions of the phenyl–thiazole scaffold, which appear to enhance the interaction with fungal targets. However, this effect remains modest compared to the reference drug and highlights the need for further structural optimization. Also, the in silico ADMET study highlights compound T9 as the most promising lead compound, showing optimal drug‐likeness, high oral absorption, acceptable solubility, and low toxicity risks.
Daoud et al. (Thu,) studied this question.