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The research aims to optimize the in vitro growth conditions of Bletilla striata using various LED light spectra.

February 5, 2026SustainabilityOpen Access

LED Light Quality Drives In Vitro Development of Bletilla striata: Toward Sustainable Orchid Propagation

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Examines how different LED light spectra optimize plantlet development in Bletilla striata, suggesting better cultivation methods.

The research aims to optimize the in vitro growth conditions of Bletilla striata using various LED light spectra.
Plantlets were cultured on Orchimax medium in 200 mL Erlenmeyer flasks.
Five different LED light treatments were applied: 100% blue, 100% red, red/blue 70:30, 50% yellow + red/blue, and 50% green + red/blue.
Fluorescent light was used as a control with a consistent photosynthetic photon flux density.
The experiment lasted for seven weeks to assess growth performance and pigment accumulation.
LED treatments enhanced plantlet performance compared to fluorescent controls, particularly with RB light promoting leaf expansion.
The highest total chlorophyll content was found under RB illumination, while monochromatic red light produced the lowest.
Carotenoids and phenolic compounds, including flavonols and anthocyanins, were significantly boosted by blue-containing treatments.
Total soluble sugars varied by organ, with red light increasing sugars in shoots and blue light in roots.

Kocot et al. (2026) studied this question.

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